Marine Microbial Ecology

How are microbes studied?

Flow cytometry

Flow cytometer output of BacLight® stained marine bacteria. BacLight® is a dual nucleic acid stain that causes live bacteria to fluoresce green and dead bacteria to fluoresce red when excited with the 488 nm argon laser of the flow cytometer. The upper coloured plot shows the dead bacterial population in red and the live in green. These populations are also shown as a contour plot (upper right). The lower plots show these populations as counts of green (FL1) and red (FL3) fluorescent particles.

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